They are easy to use and optimized for quick counting and analysis of large sample sizes with built-in analysis tools to help calibration of counts. Both plugins rely on the ability to acquire high quality micrographs with minimal background. To address this need, we developed two plugins within ImageJ for the sole task of automated hemocytometer (or known volume) and migration/invasion cell counting. ![]() Likewise, counting membranes from migration/invasion assays with the ImageJ plugin Cell Counter, although accurate, is exceptionally labor intensive, subjective, and infamous for causing wrist pain. ![]() When counting large numbers of cell samples, the hemocytometer presents a bottleneck with regards to time. ImageJ has comprehensive particle analysis algorithms which can be used effectively to count various biological particles. The National Institute of Health's ImageJ is a powerful, freely available image processing software suite.
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